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Osaka city fire department official Akira Kishimoto said the fire broke out on the fourth floor of the building located in a busy business area near the Kitashinchi train station.
The blaze affected 28 people, 27 of whom were found in a state of cardiac arrest, Kishimoto said. At least 23 people have been taken to nearby hospitals, he added.
The blaze, which broke out around 10 a.m. local time had been extinguished within half an hour, officials told the AFP news agency.
Local media footage showed dozens of firefighters outside the building, with the charred interior of the fourth floor being quite visible through broken windows.
The cause of the fire and further details are still unknown. But local media reports said police were investigating the cause of the fire for suspected arson.
This is a developing story. Please check again for updates.
rm/fb (AFP, AP, dpa)